I wanted to explore the interaction between A and A-SYN. In the first two experiments, I set up three experimental groups with three parallel samples in each group according to the concentration ratio of control, 4:1 and 8:1. a certain amount of A-SYN monomer was dissolved in PBS solution, filtered through 0.2um filter membrane, and finally prepared into a concentration of 50um. Then 300ul 50um A-SYN monomer was mixed with different concentrations of A, and 0.01%NAN3 was added, and incubated at 800rpm at 37 degrees. Samples of 5ul were taken every 12h, tested with 145ul 20um THT reagent and BioTek's Synergy™ H4 multi-mode microplate readers. The excitation wavelength was 450nm, and the emission slope length was 480nm. Take the average as the result of the time point. In the previous two experiments, I observed the inhibitory effect of A on the fiber formation process of A-SYN monomer, but the inhibitory effect of a was not significantly dose-dependent. Later in the experiment, I set up A control group,0.1:1, 0.5:1, 1:1, 1:4, and repeated the experiment in A total of 5 experimental groups. Later, I did not observe the obvious inhibition effect of A, that is, the difference between different dosage groups of A was not significant. In addition, in this experiment, I often find that the results of the three parallel samples in each group are very different, and the variance within the group is large. How should I solve this situation?
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