I have managed to get good images from negative staining grids of protein complexes (120 to 230 kDa) which seem homogenous and consistent with regards to the radii as per their molecular weights (above 8 to 15nM). Around 20-30 particles per image (49000x) and around 150 plus images also at lower magnification. I wish to carry out single particle analysis and generate 2D structure for such particles. Can somebody suggest reliable softwares with GUI (for WINDOWS platform only) that is easy to use and gives reliable output?
windows config available
-Intel Core i7-8700 (3.2-4.6 GHz, 12 MB cache)
- 16GB DIMM DDR4
- 256GB SSD + 1TB HDD 7200 obr./min
- nVidia GeForce GTX 1070
- Windows 10 Pro
Thank you in advance
Most of the free software that can do this is optimized for Unix. Why not install a free Unix version on your system? I combine the e2boxer tool of eman2 for particle picking, CTFfind or gCTF for CTF correction (can be installed within Relion gui) and Relion for sorting and averaging. If you have a direct detector you can run MotionCor2 to align the movie frames. Hope this helps.
Thank you Linda. I got EMAN2 now and trying to learn it on windows. I got your point though to use Unix based system for such analysis is much better.
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