Pankaj Thapa

5 Questions 14 Answers 0 Followers

Questions related from Pankaj Thapa

How to solve or make 2D construction maps from negative staining images?

I have managed to get good images from negative staining grids of protein complexes (120 to 230 kDa) which seem homogenous and consistent with regards to the radii as per their molecular weights...

10 October 2018 2,475 2 View

What is the acceptable amount of sarkosyl for protein purification?

I am trying to purify a protein involved in DNA repair and is a part of supra-molecular complex. This protein was over-expressed using pET28a construct in BL21 2DE3 cells but its induction was...

01 January 2018 4,964 3 View

Need suggestion for suites or tools for building the model of protein derived from X ray diffration data

I have managed to solve the structure of a protein for which X ray diffraction data was collected and using a template structure, which was only missing 13 amino acids from the N terminal side,...

07 July 2017 4,059 4 View

What is space group?

I need to understand how this link explains space grouphttp://img.chem.ucl.ac.uk/sgp/medium/sgp.htm i am unable to understand them so anybody who has a easy way out to interpret those diagrams,...

12 December 2016 3,674 2 View

List of Breast Cancer associated proteins?

I wish to carry out an exhaustive study of all the proteins which are involved/associated in/with breast cancer (associated directly or indirectly both, with regards to its progression, increase...

10 October 2016 8,135 5 View