I have precipitated bioemulsifier from a bacterial source using acetone and partially purified it by dialysis and lyophilization. The product post lyophilization is insoluble in water and many organic solvents. The solvents tested for solubility other than water were ethanol, methanol, acetone, chloroform, benzene, hexane, DMSO, PBS (pH 7), dichloromethane, ethyl acetate, octanol, diethyl ether, chloroform:methanol (1:1) and chloroform:methanol (3:1). Can someone suggest what could the possible reason for insolubility and a suitable solvent for dissolution?
Dear Swasti Dhagat sorry to see that your interesting technical question has not yet received any expert answers. Just in case that the topic is still of interest to you, please have a look at the following potentially useful article which might help you in your analysis:
Bioemulsifiers Derived from Microorganisms: Applications in the Drug and Food Industry
Article Bioemulsifiers Derived from Microorganisms: Applications in ...
Fortunately this review article has been posted by the authors as public full text on RG. Thus you can freely download it as pdf file.
Good luck with your work and best wishes, Frank Edelmann
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