Dear Hussein:

You could benefit from this valuable article about your topic at:

http://www.personal.psu.edu/mar36/stat_461/split_plot/split_plot.html.

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Indeed this article:

"ZERO-INFLATED POISSON REGRESSION | R DATA ANALYSIS EXAMPLES"

@https://stats.idre.ucla.edu/r/dae/zip/.

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Also this article " Split plot analysis in R" at the following LINK:

http://agroninfotech.blogspot.com/2018/06/split-plot-analysis-in-r-agron-stats.html?m=.

I hope it will be helpful...

With my best regards...