I have the plant extract and want to isolate the flavonoids from that. Which solvent system will be good for that.
(Subramanian and Nagarajan, 1969) for 24 hr. The methanol soluble fractions were filtered, concentrated in vacuo and the aqueous fractions were fractioned by sequential extraction with petroleum ether (Fr-1), ethyl ether (Fr-II) and ethyl acetate (Fr-III) separately. Each step was repeated thrice for complete extraction, fraction I was discarded because it contained fatty substances, whereas fraction II and III were concentrated and used for determining free and bound flavonoids respectively.
Fraction III was further hydrolyzed by refluxing with 7% sulphuric acid (10 ml/g plant material for 2 hr), filtered and filtrate was extracted thrice with ethyl acetate. All ethyl acetate layers were pooled together separately, neutralized by distilled water with repeated washings, and concentrated in vacuo. Both fraction II and fraction III were taken up in small volume of ethanol (2-5 ml) before chromatographic examination.
It depends on your target flavonoid and the property of the compounds whether it is polar or apolar. aminobutyric acid, Hexan, choloroform would be not wrong for the first testing before disolving using some solvents such as petroleum ether or ethylacetate.
Normal Column Chromatographic Separation with Hexane: CHCl3, CHCl3:MeOH solvent system, Ethyl acetate extract.
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