The phytochemical test shows the presence of steroids and terpenoids, but TLC does not show any bands. Which solvent system should I use and what should be the spraying reagent? Will the steroids and terpenoids dissolve in water?
Solvent system: Appropriate proportion of Pet Ether : Methanol : Water can be used!
According one research finding they used one solvent system:- Ethyl acetate:Methanol:Water (5:1.1:1)
Spraying Reagents:
Salkowski reagent (More used)
Liebermann Burchard reagent can be used for steroid/triterpenoids.
For reference: http://www.sphinxsai.com/Vol.3No.3/pharm/pdf/PT=23(1346-1349)JS11.pdf
Steroids are generally non-polar and may not dissolved in water...
The sulfuric acid/ potassium dichromate spray detects everything. I think it is 4% vol/ vol potassium dichromate and 40% vol/ vol sulfuric acid
Thank You
I will try with these.
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