I would like to prepare RNA from single muscle fiber for RNA sequence analysis, but I need to get rid the satellite cells. As long as I know, the method to separate the satellite cells from myofibers is by minced the muscle and treated it with collagenase type I, so it will also damage the muscle fibers. I need good single muscle fiber without satellite cells.
Have you considered using Laser Microdissection? This technology would allow you to precisely excise the cells of interest and is compatible with RNA sequencing:
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Thank you very much for Gaoyan La and Heide Marie Resch for your answer and recommendation. Actually if i want to process for RNA isolation, i separate it using RNAlater to keep the RNA content of the fiber. And i found the journal said that satellite cell will not affect very much since it will stayed in dormant condition.
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