How to separate "azadirachtin" from other limonoids (Neem seeds)?

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Dr. Prabhavathi Paulraj

Dear sir,

Thank you for gave the idea about extraction method.

Munusamy Vivekanandan

I would like to suggest two methods; one is absorbacne maximum of authentic compounds and identification of separated compounds. Second, by HPLC using C18 column and retention time. This should be more than sufficient to identify Azadirachtin