I have a sample that is going to be about pH8 and want to run SDS PAGE for Western analysis. Is this going to present any problem?
That depends on the buffer concentration. For the stacking process (isotachyphoresis) to work, the buffer system needs to be exactly defined. Too much extraneous buffer will screw this up, resulting in wide, unsharp bands.
In addition, increasing the conductivity and hence heat production in any way interferes with electrophoresis.
If you keep the buffer concentration of your sample low (say, 10 mM), you should be fine. If that is not possible, use methanol/chloroform precipitation of the sample protein to remove interfering material (doi:10.1016/0003-2697(84)90782-6).
Ideally an alkaline pH 8.0 sample shall not pose much problem during SDS-PAGE. You will definitely use a small amount of sample to mix with SDS-PAGE sample buffer which general has a pH of 6.8. As the volume of the sample is relatively smaller its run on SDS-PAGE shall nor cause any problem in the stacking gel and so does in resolving gel (usually pH 8.3).
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