How to validate regridding results of environemental data to higher spatial resolution in R?

I am working with several continous, environmental marine datasets at varying original spatial resolutions. My aim is to regrid each dataset to one common, high resolution grid. I am currently...

22 February 2021 2,290 2 View

How to do a Repeated measures analysis with not normal distributed data?

I have results from a listening test in 12 sound environments which are build up out of 3 room properties and 4 occupancy rates. Since the sound samples were often intelligible, subject scores...

04 November 2020 5,423 11 View

Can I use my DNA after the tube was "sterilized" by UV light?

I left tubes with isolated DNA drying (before resuspension) in a biosafety hood for too long and a colleague came and turned on the UV light for some 20-30min. The light did not shine on the DNA...

29 September 2020 536 3 View

How could I avoid mutations upon transforming my gRNAs into the lentiCRISPRv2 vector?

After I have cloned my gRNAs into the lentiCRISPRv2 vector and sent the construct for sequencing, I have noticed that a single mismatch between my clone and original sequence has been introduced....

11 September 2020 4,066 1 View

How do I extract mice brains for multiple experiments?

I am looking to complete western blot experiments and IHC experiments on the same cohort of mice. Is it possible to Harvest the mouse and use the right hemisphere for IHC and the left for western...

08 September 2020 5,436 3 View

DNA extraction of swabs stored in ethanol?

Hi all, I am using buccal swabs of seals as a sample source for microsats and mitochondrial sequencing. There is a few samples that were shipped stored in ethanol. , i.e. swab of the buccal area...

08 September 2020 1,013 1 View

Can anyone recommend a journal focused on "assessment & evaluation" in education that looks favourably on qualitative research?

Looking for a place to submit an article related to assessment and evaluation in physical education that was rejected clearly due to being qualitative in nature.

06 February 2020 3,229 2 View

How do I interprete the results of a moderation analysis with a categorical and a continuous variable?

Hi all, I have a question concerning a moderation analysis within an experimental setting. The independent variable is categorical and has two options. The moderator is a continuous variable. I...

06 January 2020 9,511 1 View

Can I examine chemokines using a western blot from total endothelial cell lysate?

After qPCR analysis of HUVEC lysate from my experiment, I found upregulation of several chemokine mRNA that I would like to validate via western blot. Due to the nature of my experiment, it would...

15 December 2019 5,297 3 View

Failure to detect protein by Western blotting: No expression or antobody does not work?

Dear Colleagues I have a colleague who is not expecting an antibody in her lysate to give expression of a protein at about 23kD (owing to gene methylation) However, the antibody does give bands...

11 December 2019 3,571 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View