Anyone can send the fatty acids methyl ester chromatogram with calculation method? I have doubt in C4:0 butyric acid methyl ester peak, which is showing in my chromotograme (94% area response). Anyone can give me the reference chromatogram & calculation? I am doing the analysis in GC-FID Column= Rtx 2330.
you have to clean that fatty acid (C4:0)
because it is not real fatty acid it is just solvent
so you can clean that fatty acid by change retention time,when you clean c4:0 your program will deliver concentration correctly
Some companies provide calibration mixtures for fatty acid profile in GC-FAME analysis for fatty acids.
I suggest you to low column temperature to the minimun value possible (35-40 °C, if GC is not equipped with criogenic cooling) and hold this initial temperature till the elution of solvent (C4:0 should elute at the end of elution of the solvent); then it will begin increasing of temperature for the elution of other FAMEs of mix.
- Badges
- Science topic