I was trying to separate bacteria from red blood cells by centrifugation for 1hour at 3000g in 12.5% sucrose. after centrifugation the RBC sedimented but the supernatent was little reddish (which
might be due to lysis of RBC during blood collection). after that the bacteria was pelleted by centriging at 12000g for 10 minutes. the pellet was slightly red. now while running SDS-PAGE some band of RBC proteins are observed. How to remove these red blood cell protein contamination. please suggest. any modification in the protocol is also welcome.