while doing the column , chlorophyll always makes problem for the separation
Dear
Please clarify your question. From your statement what I am getting is a separation of chlorophyll from extract, but what do you mean by column actually. SPE, HPLC, TLC or what.
Most of the time in order to check the feasibility of reaction we always seeks help from computational methods. Is it possible to check theoretical probability of a reaction through computational...
10 November 2019 2,296 1 View
Now most of the journals are not free to access research papers. Most of us now working in small college or university, where there is no free access for international journals. In order to make...
10 November 2018 2,009 13 View
Please answer, how the science will support one or several objectives of sustainable development. In many of the proposal writing procedure they asked about the same in addition to impact on...
09 October 2018 5,778 1 View
Now a days plagiarism will be the most important concern to research students who writing their thesis, manuscript and proposal. How to check the plagiarism in an effective and efficient way by...
09 October 2018 2,513 5 View
Normally oxyresveratrol found in trans form. But we can convert trans form to cis form by photochemical transformation. However the stability of cis form is too less. It can easily converted to...
08 September 2018 743 0 View
There are so many techniques are available for the extraction of natural product (Phytochemicals) like 1. Classical solvent extraction procedures 2. Ultrasound-assisted extraction 3....
08 September 2018 4,233 28 View
Normally DMF is seems to be the best solvent for my reactions. But the main problem with solvent is its dryness. Commercially available DMF is not economic. So I have to make dry DMF in my lab.
08 September 2018 5,824 0 View
There are lots of reports on multicomponent reaction involving isothiocyanate. But the generality is limited due to the availability of isothiocyanate. How to prepare isothiocyanate in an easy and...
08 September 2018 4,569 0 View
Ortho and meta C-H activation reactions are well known compared to sp3 and para C-H activation reactions. What are the suitable directing group to achieve this goal? What are best solvents for C-H...
08 September 2018 487 0 View
How to separate water soluble molecule from water ? There are some methods by adding water absorbing material. How it is possible to extract in a bench top experiment? Please give procedure for...
07 August 2018 6,638 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View