The FASTA sequence of a bacteria (Staphylococcus aureus) showed a chimeric error when submitted to the NCBI sequence submission portal.
You can't.
The chimeric sequence is the result of several errors in various step in sequence generation and you can't simply remove the error form the final sequence.
Is the data for sequencing?
Yes Nilanjana Prasad
Thank you, Dr. Abhijeet Singh, can I get any suggestions regarding this? Will having an AB file will solve this?
How many sequences do you have?
DD PCR
28 November 2023 1,895 2 View
I have synthesized Fe3O4 magnetic nanoparticles using FeCl3.6H2o and FeCl2.4H20 and NH4OH.
19 January 2023 6,726 7 View
Currently, I am using Q5® Site-Directed Mutagenesis Kit for introduction of desire mutation in genome. instead of getting single desire band, I am getting multiple band on agarose gel;. I have...
04 January 2022 5,548 11 View
I wish to know in detail about the Carbon balance for Perhydro Dibenzyltoluene dehydrogenation using Pt/Al2O3 catalyst. ? The inlet to the reactor is Perhydro Dibenzyltoluene (H18 DBT) and outlet...
21 November 2021 8,683 5 View
While running simulation with hooked udf DEFINE_SR_RATE in fluent Warning..Surface chemistry solver did not converge for NNNN cells/faces warning coming after each iterations . The number NNNN got...
06 November 2020 3,889 0 View
Kindly explain the major differences between the concept of catalyst inhibitors and catalyst poisons with suitable examples. Thanks a lot.
07 July 2018 6,786 20 View
Size range 200 to 1000 microns hydraulic diameter, square channel. Condition-Mass flux constant
07 June 2018 6,421 1 View
What additional information other than the phases present does the Raman spectroscopy generate that we could not get from XRD analysis?
16 May 2018 5,205 19 View
Kindly suggest some of the current significant topics in nanotechnology for chemical engineers.
08 May 2018 8,509 3 View
Kindly suggest some of the significant differences between the azeotropic and extractive distillation. Which one is generally preferred in the industries and why?
11 April 2018 1,330 10 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I have a .text file for various FASTA sequence , and i want to convert these sequences into a numeric file which will be in .csv format. OR I want to extract physiochemical properties(features)...
25 July 2024 3,650 2 View
I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...
23 July 2024 6,968 0 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,339 2 View
Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...
04 July 2024 9,875 1 View
Salam everyone, So I am trying to extract DNA from isolates of clinical samples of staphylococcus aureus, I am using a QIAamp UCP kit. I followed the same manufacturer protocol and used Qubit to...
03 July 2024 3,892 1 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
Dear Sir, I hope this message finds you well and in good health. I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master....
30 June 2024 8,650 0 View
Hi, everyone I would like to know if there is there any bioinformatic tool that can download batch DNA seq using imported NCBI accession numbers (upto 100 sequences) and translate them into...
19 June 2024 1,917 2 View
i am working on a virulence genes in staphylococcus and i need paper on previous and similar works
17 June 2024 5,534 2 View