after induction annealing ,we need quench the HSS from 850 degrees to room temperature within one or two hrs
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
27 July 2024 8,508 0 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
I was performing quenching studies in solution state. While plotting the Stern-Volmer graph, which intensity should I use as initial intensity (I0). For example, I take 1 ml of 0.1 M fluorophore...
25 June 2024 5,385 1 View
Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...
17 June 2024 3,434 5 View
I have prepared Copper thin films by magnetron sputtering and the samples have been annealed at 100, 200 and 300 degree celsius. The variation of the rms roughness and all other AFM height related...
11 June 2024 9,774 1 View
I am trying to do Grounded Theory in documentary research. I have obtained the most frequent terms (induction) to perform deductive coding and so, articulate a hypothesis about the hidden...
05 June 2024 2,599 2 View
I want to ask about the response of the analyte in the EIS measurement. Bare shows a high Rct response as compared to material-modified electrodes. But when we modify the material + analyte, the...
02 June 2024 873 3 View