How to quantify and differentiate As+5 and As+3 by HVG-AAS ?

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Is there any assay protocol for detection of bacterial ribonuclease P?

From a number of proteins present in crude protein extract I want to confirm the presence of bacterial ribonuclease P. Thus I want to know about any assay procedure or any other method to confirm...

04 May 2019 6,019 6 View

How to get arsenic induced protein from E.coli (using SDS-PAGE)?

Currently I am working on Arsenic induced proteins from prokaryotes. i am trying to induce proteins in the presence of sodium arsenite, but I am nighter able to get any distinct protein band in...

07 August 2017 196 4 View

Is there any method by which metal binding proteins can be detected on Gel itself?

Is there any method except autoradiography by which it can be identified that this unknown/known protein on the SDS-PAGE gel bind to a specific metal which could be Zn, Cu or As etc ?

03 April 2016 1,947 6 View

How can I get rid of zinc sulphate precipitation in LB or NB media containing Zinc sulphate at a concentration higher than 100 ppm?

Whenever I am preparing LB media containing Zinc sulphate at greater than 100 ppm concentration, it is getting precipitated. What could be done to avoid this problem as I need Zinc Sulphate at...

11 December 2015 1,706 5 View

How can I prepare solid media for long term storage of Acidithiobacillus ferrooxidans?

Is there any method for the long term storage of Acidithiobacillus species as we need to subculture it every 2 weeks?

08 September 2015 6,990 3 View

How can we check the growth of Acidithiobacillus ferrooxidans?

whether the change in the color of media to brown is the reliable one because ferrous can also be oxidized by light? Second thing is that if the waste is also producing the same color on...

08 September 2015 1,940 5 View

What are the various methods to store SDS- PAGE gels for a longer period of time (After running)?

I want to store SDS-PAGE gels that have already run. I have gone trough some methods like drying it. I want to get to know about various other methods to store gels.

01 January 1970 921 5 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View

What topic or subject does Production Engineering need to address more?

Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?

27 July 2024 648 3 View

Can i apply a public questionnaire but analyse it with a different data analysis method?

First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

27 July 2024 3,457 3 View

How to merge two or more linkage maps?

I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.

25 July 2024 6,505 2 View

Could someone please provide a list of journals that accept the application of methodologies in nature-based solutions?

I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.

23 July 2024 6,444 2 View

What effects of Autonomous Language Learning can be shown regarding linguistic competence and communicative skills?

Autonomous Language Learning can be implemented from primary to tertiary education. Practioners and students report on its effectiveness, however, there appears to be little quantitative or...

20 July 2024 2,592 2 View

Restaurant research, methods of quantitative and qualitative statistics?

Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...

20 July 2024 2,033 7 View

Franklin M. Fisher (1983) Disequilibrium Foundations of Equilibrium Economics. How is it estimated now? How is it related SMD results?

Fisher studied if there is an out-of-equilibrium process that rapidly converges to some equilibrium points. He claims that Hahn process has a Lyapounof function and therefore convergent to an...

15 July 2024 1,460 4 View

Will the leadership style used in the U.S. be successful in Australia, or will the Australians respond better to another?

Will the leadership style used in the U.S. be successful in Australia, or will the Australians respond better to another? Which leadership training methodology would be most successful with your...

14 July 2024 173 4 View

Research methodology for the evaluation of public policies?

I am looking for a recent research methodology for the evaluation of public policies. publications if possible.

14 July 2024 3,233 5 View