Is there any assay protocol for detection of bacterial ribonuclease P?

From a number of proteins present in crude protein extract I want to confirm the presence of bacterial ribonuclease P. Thus I want to know about any assay procedure or any other method to confirm...

04 May 2019 5,994 6 View

How to get arsenic induced protein from E.coli (using SDS-PAGE)?

Currently I am working on Arsenic induced proteins from prokaryotes. i am trying to induce proteins in the presence of sodium arsenite, but I am nighter able to get any distinct protein band in...

07 August 2017 129 4 View

Is there any method by which metal binding proteins can be detected on Gel itself?

Is there any method except autoradiography by which it can be identified that this unknown/known protein on the SDS-PAGE gel bind to a specific metal which could be Zn, Cu or As etc ?

03 April 2016 1,925 6 View

How can I get rid of zinc sulphate precipitation in LB or NB media containing Zinc sulphate at a concentration higher than 100 ppm?

Whenever I am preparing LB media containing Zinc sulphate at greater than 100 ppm concentration, it is getting precipitated. What could be done to avoid this problem as I need Zinc Sulphate at...

11 December 2015 1,685 5 View

How can I prepare solid media for long term storage of Acidithiobacillus ferrooxidans?

Is there any method for the long term storage of Acidithiobacillus species as we need to subculture it every 2 weeks?

08 September 2015 6,956 3 View

How can we check the growth of Acidithiobacillus ferrooxidans?

whether the change in the color of media to brown is the reliable one because ferrous can also be oxidized by light? Second thing is that if the waste is also producing the same color on...

08 September 2015 1,907 5 View

What are the various methods to store SDS- PAGE gels for a longer period of time (After running)?

I want to store SDS-PAGE gels that have already run. I have gone trough some methods like drying it. I want to get to know about various other methods to store gels.

01 January 1970 874 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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01 March 2021 3,905 5 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View