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Questions related from Shraddha Mishra
From a number of proteins present in crude protein extract I want to confirm the presence of bacterial ribonuclease P. Thus I want to know about any assay procedure or any other method to confirm...
05 May 2019 6,004 6 View
I want to quantitatively differentiate between As+5 and As+3 in media containing bacteria by HVG-AAS. Is it possible to quantify both of them separately? It would be very nice if someone can share...
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Currently I am working on Arsenic induced proteins from prokaryotes. i am trying to induce proteins in the presence of sodium arsenite, but I am nighter able to get any distinct protein band in...
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Is there any method except autoradiography by which it can be identified that this unknown/known protein on the SDS-PAGE gel bind to a specific metal which could be Zn, Cu or As etc ?
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Whenever I am preparing LB media containing Zinc sulphate at greater than 100 ppm concentration, it is getting precipitated. What could be done to avoid this problem as I need Zinc Sulphate at...
12 December 2015 1,693 5 View
Is there any method for the long term storage of Acidithiobacillus species as we need to subculture it every 2 weeks?
09 September 2015 6,971 3 View
whether the change in the color of media to brown is the reliable one because ferrous can also be oxidized by light? Second thing is that if the waste is also producing the same color on...
09 September 2015 1,915 5 View
I want to store SDS-PAGE gels that have already run. I have gone trough some methods like drying it. I want to get to know about various other methods to store gels.
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