Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View

How to adjust a SEM parameters to get a good resolution for the image ?

i synthesized pyrochlore nanopowders and i want to get a clear image using SEM techniques but oI have to know more about the parameters

12 October 2020 4,146 5 View

Why does my empty vector control fluoresce more than my fluorophore-containing vectors?

I'm using the SpectraMax M3 from Molecular Devices to read 96 well plates which contain wells with E. coli transformed with vectors containing a fluorophore under an inducible promoter. On this...

03 November 2019 1,787 1 View

Which isotope should I choose when doing ICP-MS?

12 September 2017 6,528 5 View

Can I use the quantile regression to test a interaction term?

Hey, everyone ! I really need some help for my research! Basically, my study focus on how the relationship between a and b is moderated by c, so c supposed to have moderate effect on the relation...

19 June 2016 4,639 4 View

Is there any information regarding the coefficient of thermal expansion of Panavia F 2 adhesive material?

18 March 2015 9,301 2 View

How to embed Eu-doped Y2Sn2O7 in the co-precipitation method

using a coprecipitation method, I am preparing nanopowders Eu-doped Y2Sn2O7 and I want to embed these nanoparticles, how to do that using the same method

01 January 1970 5,080 3 View

How to decrease the Z-Average(d.nm) and the PdL in Th DLS measurements and what is the right method to disperse nanoparticles ?

I am measuring the size of my nanopawder (after dispersing them in deionized water ) and every time the Z-Average and the PdL are high

01 January 1970 2,153 4 View

How to reduce the size of nanoparticles? (after having synthesized them by coprecipitation method) and how which solvant should i use for DLS

I had synthesized nanopowders by using the coprecipitation method and I got 300nm of size but I want to reduce their size for better imaging and results and also I want to characterize them using...

01 January 1970 1,789 6 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View