EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Can I performe behavioral tests in the same rats at PND30 and PND60?

Hi! Anyone knows whether I can performe behavioral tests in rats at PND30 and then, repeate them at PND60? Or this is not correct? Thanks.

20 July 2020 3,659 3 View

Load-Displacement curves with XFEM in Abaqus?

Hi everyone, I am working with abaqus by using XFEM. I am modelling a Single Lap Joint and I have simulated the crack propagation within the adhesive. Nonetheless, I also want to extract the...

18 March 2020 7,688 5 View

Anyone know the scale of tolerance of skin cells in culture, to ethanol?

I am working with cell culture and using ethanolic extracts of plants, I would like to know what percentage of ethanol from which influences the viability of the cells.

24 January 2019 8,924 3 View

DNA extraction kit for metagenomics?

Hello, I would apreciate your knowledge and answers :) I pretend to extract DNA of leaves of a plant with a lot of contaminants ! I tried before and i couldnt, even with purification! Now, i am...

24 January 2017 4,716 3 View

Phylogenetic analysis of fastq files from NGS (ion torrent) amplicon sequencing?

06 May 2015 4,487 3 View

What is a software for phylogenetic analysis (SNPs) to use on fastq files?

Hi, I want to analyse fastq files from DNA amplicon sequencing and make a phylogenetic analysis of SNPs. Can someone indicate me a good (easy?) software to do that? thanks

30 April 2015 1,566 3 View

How can i do a regression with categorical variable no binary?

Hello, i tried using the binary logistic regression in spss,the following error message is being shown Warnings The dependent variable has more than two non-missing values. For logistic...

28 August 2014 640 23 View

FACS of Trypanosoma brucei parasites?

I am interested in sort blood stream forms (BSFs) of Trypanosoma brucei. So far I've been successful sorting the procyclic form cells of this parasite using a protocol that has been published...

02 December 2013 6,462 13 View

What is the minimal amount of DNA that can be used for a qPCR reaction?

I need to do some qPCR on qDNA samples that are at very low concentrations, such as 0.5-1 ng/ul. I have very few samples left, and I was wondering if anyone knows the minimum amount of DNA that...

19 November 2013 895 17 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View