For carrying out an experiment on Aflatoxin accumulation into fish from feed, i need to produce Aflatoxin anti-nutrient in different amount in a controlled way. How I can?
More methods can use such as using U.V or fermentation by Lactic acid bacteria. In addition, improved the storage conditions to inhibition of molds growth.
First, pure culture of aspergillus flavus, spore preparation of suspension, inoculation on your material, provide a certain amount of moisture, then, it is time. After I was cultured for half a month, the toxin concentration reached 20ppb, diluted in 10ml sterile water, transferred from 50g of material, homogenized, and then added to 200g of material, homogenized. Hope it has some reference for you