How to prevent or reduce the leaky expression of only GST in a GST fusion protein?

Debjyoti Boral @Debjyoti-Boral

31 May 2021 1 5K Report

We're getting an intense 26 kD band along with our band of interest using pGEX4TI vector.

Any suggestions on how this intense band can be removed or at least reduced?

We're using BL21 Codon+ for expression.

Tapeshwar Yadav

Go through this link......i think it may help you.....Best Wishes.....!!!

https://www.thermofisher.com/np/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/gst-tagged-proteins-production-purification.html

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