How to preserve gut content from field caught insects?

25 August 2022 0 6K Report

Hi everyone

I am planning to analyse the gut contents of wild insects using metabarcoding. However, I am afraid that due to the time it takes from capture to transport to the lab, the insects will digest what they ate and I will not be able to get a representative sample of the insects' natural diet. Does anyone know of a protocol for freezing insects or other alternative so that the bacteria and enzymes of the digestive system do not break down the stomach contents?

Thanks in advance

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Diego Cruz's questions See All

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

Where can I find FDI data by sector/industry for Asian countries like India and China for the last 30 years?

Common databanks like UNCTAD, OECD, WorldBank and ASEAN Website did not have the data for the last 30 years.

09 February 2021 4,701 4 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Which statistical test to use for comparing percentages between multiple groups?

Hello everyone! I am looking for some statistical help! I am working in in vitro micropropagation and one of the aspects that I am studying is the rooting of plants in 10 different growing media....

14 January 2021 7,575 5 View

How to solve AMPS 1D simulation error?

I am trying to model a photovoltaic device using AMPS-1D software. But once I am submitting it to the queue, it remains there for an infinite amount of time without giving the result. I couldn't...

11 January 2021 9,801 2 View

What are core advantages of 2nd & 3rd generation panel data techniques over the 1st generation techniques?

I am interested to know about the difference between 1st and 2nd and 3rd generation panel data techniques.....

31 December 2020 7,204 7 View

Do chemical and physical properties influence on the size of a polymer particle?

I would like to know if the properties of a polymer impact on the size of the particle it forms. I'm working with different polyssacharides (alginic acid from different seaweed), which are...

29 December 2020 7,333 6 View

Does anyone have the Wiley Journal word Template?

Hi I need a word template file of Wiley online library because it is not available on their website.

27 December 2020 7,208 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View