Instead of the spore spread all over, I see lumps while using the haemocytometer for spore counting.
Hi: you should add tween 20 or 80 to the spore suspension ans shaking for 5 min, if it seem lumps you can go to dilution for 10-1 or 10-2 and count spore in haemocytometer.
I agree with Oadi view, To prepare the spore suspension of Magnaporthe oryzae, first of all you should add Tween 20 to the spore suspension, after that shaking for 5-10 min depends on the concentration of spores. Still you may see the lumps then go for its dilution before counting the spore.
use tween 80
prepare 2% of tween 80
sterilize/ autoclave
add 10 grams of spores
shake for 5 minutes
There you are you got your suspension
However if the spores are contaminated with bacteria then the suspension may not be sterile.
Good luck
Dear Mr. Biswal, may I suggest you, anytime you have a question like that, go to Google and then type that "question" and add "PDF" at the end (for your questions, type "How to prepare the spore suspension of Magnaporthe oryzae? PDF". You will get a numbers of PDF articles related to the keywords you have entered. Then is your job to read and pick the most relevant ones. Goodluck.
The simplest way is to overlay tween 20/tween 80 to the culture and tease the ooze a bit with the needle and then collect the liquid using a pipette. Once you have this suspension, you can filter it to remove the impurities and then you are good to set your spore concentration.