First of all you have to score your gels into binary format i.e. 1/0 ( 1 for presence and 0 for absence of allele) and then prepare your input file as below;
1. In excel sheet Cell 1A write 1 (rectangular) then Cell 1B- No of Markers (in rows) (Count allele as marker) , Cell 1C- No of genotypes (in column) and cell 1D- symbol use for missing value.
2. You can also check whether your input file is correct or not in NTSys software before your process to analysis.