When preparing stock solutions for serial dilutions for determination of molar absorbance coefficients, how can I ensure that the substance is transferred quantitatively, so that I don't introduce large errors by loosing too much substance during preparation of the stock solution? What is the best method to prepare these solutions? How to best transfer the weighed substance into the liquid?
You can follow this general recipe:
1) weigh the solid in a glass vessel.
2) dissolve it in a small volume of distilled water.
3) carefully transfer the liquid to a volumetric flask.
4) rinse the vessel with distilled water and transfer to the volumetric flask (repeat this step several times).
5) Adjust the final volume.
Of course, the balance and the volumetric flask must be properly calibrated.
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- Methodology
- Absorption Spectroscopy