Hi all,
I am trying to make a standard curve for Nile Blue A fluorescence using Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) as the standard and a plate reader to measure fluorescence. My issue is, I have only managed to solubilise the PHA polymer using chloroform, which then also solubilises the polystyrene plate I am using for analysis. In addition to this, chloroform does not mix well with the Nile Blue A solution I am using to stain with, which is mostly water. So my question is, is there a more non-polar solvent I can use to dissolve the PHA polymer that will not immediately effect the plate, or alternately, if I grind the polymer strips down into a powder, will the Nile Blue A still be effective at staining this?
Thanks.
Dear Karri Thomas, still your question is not clear enough for me. Please check the following RG free documents, hope they will be usefull. My Regards
https://www.researchgate.net/publication/13375438_A_sensitive_viable-colony_staining_method_using_Nile_Red_for_direct_screening_of_bacteria_that_accumulate_polyhydroxyalkanoic_acids_and_other_lipid_storage_compounds
https://www.researchgate.net/publication/304612344_Methods_for_identification_quantification_and_characterization_of_polyhydroxyalkanoates
Dear Karri Thomas, still your question is not clear enough for me. Please check the following RG free documents, hope they will be usefull. My Regards
https://www.researchgate.net/publication/13375438_A_sensitive_viable-colony_staining_method_using_Nile_Red_for_direct_screening_of_bacteria_that_accumulate_polyhydroxyalkanoic_acids_and_other_lipid_storage_compounds
https://www.researchgate.net/publication/304612344_Methods_for_identification_quantification_and_characterization_of_polyhydroxyalkanoates
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