I am using an alcohol extraction( etanol 99% ) with soxhlet, but how could I stop losing medicinal terpenes and flavonoids and and decrease the chlorophyll content ?
You can not loose 100% terpenes.finish your extraction and carry out phytochemical screening,you would discorver my point.
Is alcohol extraction ( etanol 99% ) with soxhlet, better for the extaction, if you are interested in terpenes content of your medicinal plant material?
you can use , successively , 3 solvents of increasing polarity. In first , extract in an apolar solvent, then in a solvent that is not very polar then use the apolar solvent
A good rule in dissolving: like dissolves in like. Therefore, use for extraction, for example, pinens.
Hi,you can remove the chlorophyll by extracting in petroleum ether.Then do the Soxhelt extraction with solvents of increasing polarity.
Terpenes are highly non-polar compounds. So Soxhlation with highly polar solvent like Water or Methanol would not likely extract the non-polar Terpenes. So, extraction with any non-polar solvent like Per-ether or n-Hexane would help.
Use a non-polar solvent like n-hexane and a process that does not involve high temperatures as this may vaporize or decompose terpenes.
Make use nonpolar a solvent. Then try using rotary evaporator for concentration. The principle applied is low temperature/high pressure.
You will get terpenes in n-butanol fraction of the
(aquous and alcoholic) plant extract!
If you have volatile terpenes in your plant, first you might extract your plant by hydro-distillation using Clevenger so to obtain volatile oil terpenes, get rid of any wataer residues using sodium sulphate. Second, you can extract the rest of none volatile terpenes with ethanol and n. Hexane. Following centrifugation, filtration and getting rid of any chlorophyll, you will get a second pure extract. Finally identify all the compounds from both extracts, after running your pure extracts against standard terpenes with GC - MS.
Read this book "Plant Drug Analysis: A Thin Layer Chromatography Atlas"
By Hildebert Wagner, Sabine Bladt
chapter 7 for flavonoids and chapter 15 for terpenes
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