Software flowjo and flow cytometry. How to use?

Is there anyone that understands about flowjo software and flow cytometry? I need help to use this software.

02 December 2020 9,996 8 View

Does someone have tips on how to reduce the amount of entrapped oil in the membrane of GUVs with the Phase Transfer Method?

I'm trying to prepare GUVs using the phase-transfer method but apparently I'm getting residual oil entrapped in the bilayer. Did anyone manage to overcome this? Basicaly, I'm following the...

24 November 2020 2,962 1 View

How do you delete a Google Scholar profile?

For some reason, I have two slightly different names associated with my Google Scholar profile. Apparently, both names are associated with the same email. I tried to eliminate one of the names,...

21 October 2020 9,396 3 View

Using facebook for surveys?

Hi, Has anyone had any good (or perhaps bad experience) using a "boosted" survey on Facebook? Has anyone been able to publish using this channel for data collection? Or has anyone been...

12 October 2020 6,697 4 View

Someone has a suggestion to improve the purification of MMLV-RT protein?

I am working with a MMLV-RT (Moloney murine leukemia virus- Transcriptase Reverse). I am expressing in E. coli pTGroE and purifying in a Chelating Sepharose loaded with Ni2+. I am eluting the...

01 June 2020 4,105 4 View

How can you insert measuring items in Projective Techniques?

I am conducting research on consumers' attitudes (namely trust & skepticism) towards Corporate Environmental Responsibility communication and would like to use Projective Techniques (Psychology...

22 March 2020 7,527 3 View

Is it possible to calculate pIC50 of a plant extract?

Is there a way to calculate the pIC50 value of a plant extract? As far as I know, in order to calculate pIC50, the IC50 must need to be in molarity, therefore, it's not possible to get the value...

08 January 2020 8,540 3 View

Why do my protein samples and standards get stuck running Western blot?

When I'm running an 8% SDS-PAGE gel at 100 V, my samples and standards get stuck after a while. Does anyone know why this is happening? Thanks!

20 May 2019 7,797 3 View

Hi, I would like to solubilize a hidrophobic compound in order to administer it to mices. Should anyone suggest a nontoxic vehicle?

The compound is very insoluble in aqueous solution and the compound administration will be given orally. The compound manufacturer has no information regarding the solubility of the compound....

16 January 2019 834 1 View

Anyone can recommend an efficient method for sterilization of corn seeds?

I tried ethanol 70% for 3 min, then bleach 5% for 3 min, followed by 3 rinses of sterile water. Also, tried the same method, but instead of 3 min used 6 min.

10 October 2018 6,640 11 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

Is carboxymethyl cellulose a better electrolyte host polymer than methyl cellulose and cellulose acetate?

I want to prepare a cellulose-based biopolymer solid electrolyte by solution casting technique. However, choosing the right cellulose derivative has been my major challenge. Among the many factors...

30 January 2021 5,355 3 View

What is the mechanism or interaction between Fe3O4 magnetic nanoparticles and biochar?

Lately there are many studies on biochar incorporated/loaded/coated with Fe3O4 magnetic nanoparticles. But I havent found any paper that describes the interaction or bonding between the two. How...

20 January 2021 3,162 4 View

Why does Reaction Wood acts opposite in gymnosperm and angiosperms?

I am wondering why the Gymnosperms have an opposite behaviour that Angiosperms in the Reaction Wood (RW). Are there some new findings regarding that? Studies say that causal agents can be the...

10 January 2021 8,744 4 View

After cellulose is regenerated in water, a cellulose hydrogel is formed. I want to know if there is crystallinity inside the the hydrogel?

After cellulose is dissolved and regenerated in water, a regenerated cellulose hydrogel is formed. I want to know if there is crystallinity inside the hydrogel, or it will not crystallize until it...

04 January 2021 7,466 4 View

How to calculate "POWER LAW" in rheology from a GRAPH of shear rate vs viscosity for cellulose nanofibrils suspensions?

How to calculate "POWER LAW" in rheology from a GRAPH of shear rate vs viscosity for cellulose nanofibrils suspensions?

02 January 2021 6,816 3 View

What are some of the applications of transparent wood?

Are there any particular uses for transparent wood other than visual appeal? The transparent wood is really cool to see, but what are the practical uses? Please provide examples. Happy New Year!

31 December 2020 2,479 4 View

Crystallinity index measurement of Cellulose nanocrystals ??

How to find the crystallinity index of cellulose nanocrystals using XRD peaks. Can anyone suggest the most appropriate method of finding CI

28 December 2020 7,809 4 View