First fresh flowers, leaves, fruits or seeds have to need collect and washed thoroughly with distilled water several times to remove the dust and dried under shade. About 15, 25, 50 gm or more collected plant cut into small pieces and kept in a flask containing 50, 100, 150, 200 mL, or more distilled water. That boiled 70, 80, or 90oC for 40, 50 or 60 min (its depend on mixing with water) with stirring to produce an extract. The extract was cooled to room temperature and filtered. Finally, the extract was cooled at 4°C and stored for further synthesis

To prepare leaves for plant extraction need to collect leaves, fruits, seeds, fresh flowers then washed several times by distilled water to eliminate the dust and dried under room temperature. After that the collected leaves, fruits, seeds, fresh flowers have to cut into small pieces and kept in a flask containing distilled water. Then boiled with continues stirring to yield an extract. Lastly, the extract have to cool at 4°C temperature.