Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How or where can I get the crystallographic information file (CIF) for PCN-222 MOF?

I need the simulated XRD pattern of PCN-222 to compare with experimental data. Thanks.

19 February 2021 4,932 3 View

Websites for virtual lab in semiconductors?

Hello everyone, Because of Covid, now all classes are online. Is there good website that also are easy to use to teach student virtual experiments about PN junctions and semiconductors? Thank...

24 December 2020 6,637 2 View

From the EDAX analyses if the material which is coated is not observed in maximum percentage then how can interpret the results?

If we doped metal oxide to semiconducting material in different ratios then the property of the resultant material will be increased.But in some lower ratios when we go through the elemental...

16 December 2020 8,190 2 View

Is there any software for calculating hit bias (HB), missed precipitation (MP), and false precipitation (FP)?

I have a time series of two rainfall products. For error decomposition analysis, I have to calculate HB, MB and FB. Is there any software for calculating these variables.

27 November 2020 980 2 View

Why Schiff bases change colour upon evaporation?

I got bright yellow coloured Schiff base after 6 hour reaction time. So i kept it for slow evaporation. Solvent was methanol. But after complete evaporation my product colour changes from yellow...

17 November 2020 3,824 2 View

How to make the children cool, who feel stressful during this pandemic-lock down without having physical outlet?

The discussion may feed the parents a way to manage and guide the children in a right direction.

12 November 2020 1,302 9 View

How to design qPCR primer for mature microRNA?

09 November 2020 9,163 3 View

Which force field should be used to simulate protein-protein complex in gromacs?

Hi, I have done a protein-protein docking. Now I want to perform MD in gromacs. I am confused with forcefields. When I tried to findout, everywhere it is written for protein-ligand but I couldn't...

29 September 2020 1,410 3 View

Charcoal mediated steroid depletion assay?

Suppose, one is doing enzyme assay of an enzyme of a steroidogenic pathway. Is it necessary to deplete the steroids from the tissue/ cell homogenate before administering substrates to check enzyme...

21 September 2020 7,439 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

Could someone recommend papers about PSO technique?

Hi, everyone Could someone recommend papers about Particle swarm optimization (PSO technique), using MATLAB, please? Thanks in advance

03 February 2021 3,811 6 View

Any method or procedure of parameters estimation for epidemiological mathematical modeling?

As in the image below, may someone help us on how to estimate the parameters used on epidemiological mathematical modeling described in systems of non linear differential equations? Any possible...

22 January 2021 2,869 3 View

What is the best software for storm water flood inundation mapping?

I'm modeling a storm event hydrologic responses of urban watershed using fully distributed hydrological model? In this modeling I want to generate the spatially distributed runoff values in the...

20 January 2021 5,343 7 View

Do we need to keep looking for the fracture angle after the Puck's failure initiation is reached?

Greetings to everyone, Our team is currently working on the development of a VUMAT code in Abaqus for composite modelling using the Puck's failure theory as damage initiation criteria. Currently...

20 January 2021 6,367 1 View

Which model is most suitable for urban simulation?

Respected sir/mam, I want to simulate the urban expansion using different time series LULC based on satellite image. please suggest me most suitable model for urban simulation. Thanks and regards.

17 January 2021 9,134 11 View

Is there any analytical model to asses impact of rigid projectile on monolithic deformable plate?

I am trying to select suitable element type (Solid or Shell) for impact analysis. I have tried with both solid and shell elements. The results are same for both element types up to certain impact...

08 January 2021 3,251 4 View

Which software and classification method are most appropriate for land use land cover classification?

Respected sir/mam, I have using satellite image of Landsat 8 and 5 for LULC classification. I am confused that which software and method are the best for LULC classification? Please help me....

07 January 2021 3,884 20 View

How to develop mathematical model for Eye-Gaze tracking system?

Gaze tracking technologies are used in a wide variety of applications. Most gaze tracking systems require a personal calibration process where the user is instructed to gaze at certain points...

03 January 2021 9,675 5 View