Ionic cross-linking method and then optimized by using Box-Behnken response surface method (BBRSM).

Wishes

Check this article for conventional deposition

"Chemical characteristics of O-carboxymethyl chitosans related to the preparation conditions"

Carbohydrate Polymers 53 (4), 355-359, 2003

For a more discrete control of nanoparticles you could go for physical vapordeposition methods with masking

Dear Priyanka,

For preparation of carboxymethyl chitosan nano particles you can use

01. simple ionic cross-linking using TPP and CaCl2,

02. cyclic freezing/thawing technique,

03. γ-irradiation method, etc.

I hope I have answered your question.

With Best Wishes,

Samir G. Pandya