My bacterial suspension volume is 10 microlitre only so i cant see any pellet if i do centrifugation
https://www.jove.com/video/3064/aseptic-laboratory-techniques-plating-methods
This small volume can be used for TEM. Mix the pellet volume in about 100 microliters of 1% phosphotungstic acid prepared in PBS pH 7. Load this volume on a TEM grid, bearing formvar coating, keep for 30 min, soak excess volume of liquid with a filter by touching edge of grid. Dry the grid and view in TEM.
Dear Siva,
the suggestion by Prof. Shamsher SK is accurate. 100 uL is sufficient to perform a TEM imaging for your sample.
Thank you Dr. Kanwar and Dr. Navnith kumar. Sir thanks for your valuable suggestions. I will try this method and i hope i can get good results thanks a lot
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