How to prepare amino bromomethane?
By adding Br2 to MeNH2.HCl in EtOH at 10-15° under inert atmosphere and the mixture stirred 5 h at 40° to give 90.5 mol% BrCH2NH2.HCl
Good luck
Thank you Sir.
poultry production in nigeria
29 July 2024 5,518 2 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Trouble 1: I ran a PCR reaction and found a low molecular weight band (100 bp) when doing electrophoresis. 1ng plasmid (OD260/280=1.84) was used as template. Final concentration of primers was...
13 June 2024 5,540 6 View
Good morning, I am currently engaged in research in the field of virtual reality. I would be grateful if you could share your opinion on the subject. Your input is invaluable to me. Kind...
25 May 2024 8,567 5 View
I want to detect my target protein through WB and it worked well before. But now I could not get any signal for both my target and the GAPDH, so I ran SDS-PAGE. I expected many bands on the gel of...
23 May 2024 613 6 View
I want to copy a target gene from cDNA into a plasmid. The primers were designed according to the CDS sequence from NCBI. But when I performed PCR reactions I could not get any target bands. So...
15 April 2024 2,115 4 View
I found to use a wrong secondary Ab for my WB when I visualized it with ECL, can I wash with TBST for several times and incubate the right secondary Ab? Another question is why my bands are not...
16 February 2024 3,187 4 View
👋 Sto attualmente conducendo una ricerca finalizzata ad esplorare come le attività di employer branding possano permettere alle imprese di garantire una EVP (Employee Value Proposition) di...
17 January 2024 9,183 1 View
When I overexpressed a protein in a cell line, I found decrease of phospho-PLC (phospholipase C) at the protein level through WB. But when I knocked out the protein, there was no difference of...
10 January 2024 8,201 3 View
I want to use the Cre LoxP to knock out my target gene in a mammalian cell line. According to the mechanism, I need to add two loxP sequences at both ends of the gene through homologous...
21 November 2023 5,133 1 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?
28 July 2024 5,331 1 View
how to separate the organic reaction stereo isomers compounds are separate from the reaction mixture any simple methods are available
23 July 2024 8,025 4 View
Thanks
22 July 2024 1,143 3 View
Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...
21 July 2024 2,476 2 View
One has to understand about the enhancement procedures and treatment systems I can list around 12 procedures which are tried and tested and are proven so let us see how many in total the experts...
20 July 2024 3,392 2 View