im try to do decellularization using dnase1 but I'm not getting the correct result as required. there is some issue in my calculation I guess. can anyone help?
Some research indicates that the fist steps toward democratization of an authoritarian regime actually lowers state capacity. How strong is the evidence for this hypothesis?
14 July 2024 8,239 3 View
im working on the photocrosslinking of hydrogels and want to check whether crosslinking happened with the material I have.
12 June 2024 5,579 3 View
im working on hydrogels now and I would like to use RF as a photoinitiator
24 May 2024 9,131 1 View
microplastics in our environment
18 February 2024 374 0 View
How to check pH of PCR mastermix?
30 November 2023 1,413 3 View
Please provide for me the procedure to upload a new text.
02 November 2023 1,239 0 View
I have a question about the relationship between the cross-sectional area of an electrical transformer and the maximum power it can deliver. Empirically, the area is calculated by the square...
09 October 2023 1,259 4 View
I am trying to transfer a group of bacterial strains but they loose the plasmid in the down stream processes. Iam wondering if i can be to transform them in a way that the gene of interest gets...
25 September 2023 1,160 2 View
I was uploading a chapter and before I filled in the book, the chapter got posted with no context and I cannot find a way to go back and add the remaining details. I would be happy to start over...
04 August 2023 6,511 0 View
I will estimate the h2 of a trait in pigs using a model with common environmental effect in addition to fixed, additive and residuals, can anybody help me in doing this in SAS proc mixed (REML)?...
21 July 2023 5,608 0 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...
14 July 2024 641 1 View
I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...
27 June 2024 2,951 5 View
Hi, I want to obtain circular RNA and lariat. I need treat my samples with DNase I and RNase R. Do you know, I can do this in the same time? And maybe do you have any protocols for simultaneous...
02 June 2024 9,544 0 View
Hi, I am learning to perform footprinting experiment. In the protocol I have there are the following components of the reaction buffer: HEPES buffer (7.8 pH), glycerol, BSA, DTT, KCl, MgCl2,...
28 May 2024 1,317 0 View
Hello, everybody. I am looking to add a DNAse treatment step to my bacterial lysis (gram-negative) procedure for crude enzyme extraction because my lysate always ends up being too viscous. We...
14 April 2024 280 2 View
Hello everyone, I am working on the production of IVT mRNA. After transcription, the remaining DNA templates are digested by DNase I. I have tested transcription with uncut DNA (mostly...
10 April 2024 1,362 2 View
I am using the Thermo Scientific DNase I (https://www.thermofisher.com/order/catalog/product/EN0521) to remove genomic DNA from RNA extracts eluted in nuclease free water. The protocol calls for...
30 March 2024 7,100 0 View
I've been trying to extract RNA from mouse lung tissues (normal and tumour) and slowly improving the yield and purity measured via Nanodrop and Qubit (I'm consistently getting 260/280 ratios of ~2...
21 March 2024 113 5 View