The following data is a GC-MS profile of a fungal extract (DCM is used as a solvent for extraction). Colour is rose-red in Acetonitrile, and the red compound is highly polar, soluble in water and other polar solvents, practically insoluble in nonpolar organic solvents. the colour is shifted to yellow-red in acidic condition and purple-blue in basic condition (see the picture). The colour changing behaviour is similar to Doxorubicin, but I am unable to analyze GC-MS data. How can I predict the compound from the GC-MS profile?
GC sample was prepared as follows: DCM extract dried, add 80 µL Methoxyamine-pyridine (1%), heat 60 min at 60° C, add 80 µL MSTFA, heat 30 min at 60° C, kept 4° before running in GC. The sample was run the next day.
Firstly try to purchase pure compounds or working STD made at Ur lab, to confirm & to compare the graph at different levels to avoid confusion arised due to different conc and detector response.
The confirmation can be made by comparing the unknown spectrum and the library match.
Before confirming by library match, consider these points carefully.
a) The library show only data of compounds stored in its software.
b) If u put cursor on any peak of GC-MS chromatogram it will display list of compounds with number display like 95, 90, 85 & 65. These number display indicates the % match with Ur unknown compound (anything above 90 is considered as best match with Ur compound).
c) In addition, match the displayed peak pattern with ur unknown compound like major ions matching with ur unknown spectrum, if missing u don’t have a match.
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