Is there any other software to plot TADs ( I have chromosome start and end position), normalized tag count from ChIP-Seq data, and log2FC of genes (fold change from RNA seq data) in one graph?
I think you can do this with a program mathematica!! If you can not tell me
I have used CycloPs and ACD/chemsketch to generate virtual structure of peptide (pdb format). Is it necessary to optimize the structure or do energy minimization studies before proceeding to...
10 November 2013 6,113 5 View
My peptide is not getting fragmented by ESI-MS. The peptide mass is below 1kDa. I have already checked for Cys residue. I have not pretreated my sample with enzyme or 6 N HCl. Is there any...
08 September 2012 1,791 4 View
My separating gel is not getting polymerized. (It does not set). I am following protocol by Schagger, Von Jagow protocol. Can anyone please forward me your protocol for Tricine SDS PAGE?
07 August 2012 8,079 7 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...
03 August 2024 6,070 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...
31 July 2024 9,697 0 View
The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...
30 July 2024 3,126 0 View
Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.
29 July 2024 4,971 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View