I Tried HPLC of the sample with the following set of parameters set:
Instrument: LC AD 20
Flow rate: 1ml/min
Temp: 30 degree
Sample Injection: 30 microliter
Protein analyte conc: 5mcg/ml, 10mcg/ml, 20mcg/ml, 30mcg/ml.
Time for running: 15min
Pressure: 4293 Psi
Solvent used: 30% Acetonytrile(ACN:D.H2O)
Prior to the use I washed the column with solvent for 1 hour and found that the peaks were not clear and same upon repetetion. Can anyone suggest how to perform HPLC for a short aminoacid sequence(11 aminoacids) structure.
Thankyou