Is it possible for aqueous fraction to have more a-amylase and glucosidase inhibition than EtoAc fraction and MeOh extract ?

I am working on inhibition of both alpha amylase and glucosidase by Methanolic extract and its sub fractions of EtoAc and Aqeous resin fraction. I have sub fractionated EtoAc fraction with resin...

11 December 2020 5,926 2 View

How to perform correlation testing between two datasets in which one dataset has gender-specific reference ranges?

I want to test for correlation between dataset A and dataset B (both have numerical data). For example, dataset B consist of hemoglobin values which have different reference ranges for male and...

20 October 2020 9,550 6 View

Is information only an abstract, subjective, mathematically well-formalized concept, or is information a kind of concrete natural substance?

Perhaps, in our uncertainty time, the importance of the concept of "information" in any area of human activity is obvious. For many decades, publications on the theory of information and its...

23 May 2020 4,639 3 View

Digital Workflow Methodology for BIM enabled infrastructure projects?

Hi, What are key journals in Digital Workflow Methodology for BIM enabled infrastructure projects? Thanks

15 April 2020 5,313 1 View

BIPV architectural requirements?

Hello, im student from slovakia.I am writing a bachelor thesis on BIPV photovoltaics. I would like to describe the architectural requirements. Can you give me an article where I would read about...

01 April 2020 5,888 1 View

BIM framework for fire safety design?

Can you recommend good publications on development of integrated BIM framework for fire safety design?

24 March 2020 5,569 3 View

Can anyone recommend good process optimisation algorithms and publications?

Can anyone recommend good process optimisation algorithms and publications, for example to improve productivity of structural design workflow?

24 March 2020 2,306 3 View

Possibility of group comparison in hybrid choice models with Mplus?

Greetings everybody, I have data from a survey including a discrete choice experiment. Overall the data contain 5 treatments groups (5 subsets). Then I have developed a hybrid choice model with...

19 February 2020 2,311 3 View

The current precarious situation of fundamental theory-based educational reflection?

Dear colleagues, among other things, in discussions at the American Association for Moral Education Conference in Seattle in 2019, colleagues and I shared the impression that research in social...

24 November 2019 5,206 3 View

No title

26 July 2019 3,331 8 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View