I am using a Proxeon NanoLC and currently using 25 cm long column. I am interested in equipping the machine with a 50 cm column as shown in some posters from Thermo. Does anybody know how to pack a 50 cm column and the equipment needed for packing?
I am interested in a compound that can enhance memory or protein memory. We don't have access to mice or other animals, so does anybody have any suggestions for cell models or methods that are...
03 April 2016 1,463 1 View
X-Ray and NMR can both elucidate protein structures. Are there any major difference in sample suitability between these two methods? What are the advantages and disadvantages of each method?
01 February 2016 8,937 3 View
When purifying enzyme or a specific protein using FPLC with all kinds of column chromatography. How to test the protein purity in the following two cases, authentic standard available and...
10 November 2015 6,194 8 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
How can I select cells in prime editing without fluorescence-activated cell separation?
31 July 2024 8,551 2 View