I am using a Proxeon NanoLC and currently using 25 cm long column. I am interested in equipping the machine with a 50 cm column as shown in some posters from Thermo. Does anybody know how to pack a 50 cm column and the equipment needed for packing?
03 April 2016 1,421 1 View
01 February 2016 8,890 3 View
When purifying enzyme or a specific protein using FPLC with all kinds of column chromatography. How to test the protein purity in the following two cases, authentic standard available and...
10 November 2015 6,147 8 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
03 March 2021 5,360 2 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...
26 February 2021 3,738 3 View
Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...
26 February 2021 4,026 5 View
In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...
26 February 2021 3,806 2 View
Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!
25 February 2021 3,092 2 View
I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...
25 February 2021 8,039 1 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View