How to optimize my ChIP protocol for FFPE tissues?

02 March 2022 0 762 Report

I am performing a ChIP protocol using the iDeal ChIP-FFPE kit by Diagenode (C01010190) to isolate histones from FFPE tissues. After the immunoprecipitation I didn’t obtain a quantifiable DNA concentration, and in the western blot control I couldn’t see a band corresponding to my immunoprecipitated target.

Any tips for optimizing the protocol?

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