01 January 2018 1 9K Report

I would like to do Fluorescence-activated cell sorting (FACS) on differentiated cells that were grown on the transwell inserts. These differentiated cells will die quickly after being removed from the monolayer they are growing on so I want to "wash" them off the membrane and "fix" them as soon as they are off the transwell. Is there an optimal way to do this? Has anyone read publications that performed FACS on cells in transwells?

transwell: life sciences Costar 3470 transwell plates

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