Leon Hsieh

3 Questions 4 Answers 0 Followers

Questions related from Leon Hsieh

How to optimize FACS staining/fixation for cells in transwell insert?

I would like to do Fluorescence-activated cell sorting (FACS) on differentiated cells that were grown on the transwell inserts. These differentiated cells will die quickly after being removed from...

01 January 2018 8,739 1 View

What's the best way to grow A549s on agar gel?

Hi, I am conducting experiments on growing A549s on agar gel. I am using RPMI with HEPE as my media. My problem is the A549s seems to proliferate much slower compared to those grown on tissue...

11 November 2015 3,416 7 View

How does IL-1b get secreted in in vitro inflammasome reconstitution systems?

Hi all, In vitro inflammasome reconstitution system is common in studies involving NLRP3, NLRP1 etc. Some examples of murine and human NLRP3 inflammasome reconstitution is linked...

01 January 1970 7,894 1 View