We are interested in RNA isolation from insect intestine. Actually we use Trizol and we obtain a lot of contaminants (protein and maybe carbohydrates). After phenol/chloroform purification, contaminants still, but RNA is not clearly visible.
We would love to have a reliable protocolo for RNA isolation. Any suggestions? thanks in advance.
Gustavo
http://www.sciencedirect.com/science/article/pii/S0888754312000274
Thank you very much for your suggestions, greatly appreciated.
We make sure of RNA preservation by using RNAlater, so this should be no problem. We will try suggested kits and DNAse treatment of course.
Later on will post some news. Thank you very much again, please receive my best wishes and apprecitation.
Gustavo
You can precipitate RNA several times by LiCl until it gets pure.
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rna-isolation/general-articles/the-use-of-licl-precipitation-for-rna-purification.html
It helped when I was purifying total RNA from a beetle with Trizol.
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