I have been trying hard for a month to observe some definite structure of the biopolymer which was synthesized by our lab. The irony is it cannot be dissolved in any organic solvent and we are left only with some aqueous buffer for our studies.
Since SEM requires solid samples i highly doubt to see anything under it except for some random aggregates.
Even in regular TEM I can only see indefinite aggregations of polymer. I have tried staining as well which in some instances effected polymer structures, like i have seen some nano vesicles but those were not the representative of the sample.
Please dont recommend cryoTEM because we are struggling hard to get the dates, but chances are not likely.
Need Help!
Dear Dr. Zeeshan Uddin,
I suggest you to have a look at the following, recent paper:
-Microscopic Techniques for the Analysis of Micro and Nanostructures of Biopolymers and Their Derivatives (Review)
Abhilash Venkateshaiah, Vinod Vellora Thekkae Padil, Malladi Nagalakshmaiah, Stanisław Wacławek, Miroslav Černík, Rajender S Varma.
Polymers 12(3):512 (2020)
Available on RG at: https://www.researchgate.net/publication/339539840_Microscopic_Techniques_for_the_Analysis_of_Micro_and_Nanostructures_of_Biopolymers_and_Their_Derivatives
and at: www.mdpi.com (in pdf format).
Best regards, Pierluigi Traverso
- Badges
- Science topic
- Similar topics
- Chemistry
- Nanotechnology
- Nano