How to monitor half life of peptides?

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Does anyone has a standard PK/PD protocol?

Can any one provide me a standard PK/PD protocol . for small molecule/ peptide?

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CD spectra plotting for DNA

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Has anyont faced a problem with making/stabilization of G4 oligo for smFRET?

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11 December 2016 8,846 2 View

Why am I not seeing a sharp peak in smFRET?

I am trying to see a G4 in an smFRET experiment. Although I am using 150mM NaCl and getting a FRET peak at 0.8 but the peak is diffused through 0 to 1 and not a sharp peak. Any one has any idea...

11 December 2016 1,181 8 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

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How to analyze multiple phosphorilation sites?

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Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

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31 July 2024 6,544 11 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

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How to cacualte the creep life of polycrystal with CPFEM?

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Does sucrose interfere with the BCA reagent in peptide concentration assays?

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What strains of C57 mice can induce rheumatoid arthritis?

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09 July 2024 6,657 0 View

I found trouble to run MD of my peptide with PTM in different force field. In Charm-GUI, it cant recognize the engineering residue. What can I do now?

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How to prepare a transparent solution of the peptide with the mixture of hydrophobic and hydrophilic amino acids ?

I am using a peptide and have faced challenges in preparing a transparent peptide solution. My goal is to prepare a peptide solution in MES buffer and then use the solution to immobilize the...

20 June 2024 8,622 5 View

Guobing Xiang

Anukana

Half live for(in) what kind of process?

Sorry for asking!

Andrei Blasko

For biological activity you need a bio-assay method. For chemical and physical degradation, PRLC, SEC, CEX and CZE chromatographic methods.

Rob Keller

Dear Anukana,

While being busy on something else I ‘stumble’ on the following site called PEPLife:

http://crdd.osdd.net/raghava/peplife/

Select the download option and subsequently reference:

http://crdd.osdd.net/raghava/peplife/ref.php

Here you find references to numerous studies. Possibly you find a desired and for you useful procedure.

Good luck!

Anukana Bhattacharjee

Rob Keller This looks really interesting and useful. Thanks!!!