Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Contamination when culturing primary uterine endometrial cells?

Dear everyone I'm working on the endometrium of the uterus. I need to extract endometrial cells from the endometrial tissue. Usually, I will separate the tissue into 2,3 mm pieces and incubated in...

25 November 2020 7,428 3 View

What are the morphological changes of PC3 cells during autophagy and/or apoptosis?

I was performing wound healing assay seeding 300,000cells/well in 6 well plates with 2mL RPMI medium. At 0hr after intervention, the cells looked like the first picture. At 24hr after...

18 November 2020 9,671 2 View

Does PROCESS v3.5 by Andrew F. Hayes with SPSS Statistics 27 on MacOS 10.15.7 work?

After installing the PROCESS v3.5 tool, I ran a mediation model 4 with three variables (N = 125). The warnings below showed up on the output window, and the status at the bottom shows, "Running...

15 November 2020 3,930 14 View

How do you deal with massless particles in SR, since they can't have four vectors?

Suppose a photon is observed to have some three velocity v in an inertial frame O, another inertial frame O' is moving away from O with another three velocity u. Is there a general way to get the...

10 October 2020 5,132 3 View

What are the peaks at 14.3 and 28.4 in LDH's XRD pattern?

Hi! I'm synthesizing MgAl-NO3-, MgAl-Cl-, MgAl-CO32-, etc. by coprecipitation method using metal nitrate salts, NaOH, and anion source coming from sodium salts. Besides those characteristic peaks...

21 September 2020 3,402 3 View

The correctness of synthetic data and corresponding label generating from smote or adasyn ?

Dataset imbalance is a major problem usually encounter in machine learning practice. Some technique such as SMOTE or ADASYN generate addition data (synthetic data) to avoid imbalance problem....

06 September 2020 3,337 3 View

PC-3 cells: what does a neuron like/threadlike morphology indicate about the cell's condition?

I was performing wound healing assays with PC-3 cells (cultured in RPMI+10%FBS; 500,000 cells/well in 6 well plate). I seeded them for 24 hours, then applied the scratch with a 100uL pipette tip....

03 September 2020 7,664 4 View

Do you have any references on "Process to develop Child centered Social accountability"?

Dear all , I am working as the consultant for Save Children International to develop and pilot a process named " Child Centered Social accountability" in Vietnam. Does anyone know where I can get...

31 August 2020 6,297 5 View

How to build up a cell model for endometrium receptivity?

I want to conduct some research on the topic of endometrium receptivity, but I only have HEC1A cell line available. Can you please share with me the experience in using which cell line for this...

26 August 2020 2,381 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View