Hi, I wanna know what NaOH type (ready-made solution/ Pellets) y'all use in denaturing libraries when using with MiSeq V2 nano kit and the pH value?

I use a MiSeq machine with MiSeq V2 Nano kit. There isn't any pH value that NaOH should be adjusted to, But I'm wondering if that can cause errors in the sequencing run. Thanks! Eranga

11 June 2018 7,113 2 View

How to do NDVI analysis and data collection?

27 September 2017 1,293 3 View

What kind of metrics can I use to compare two 3D surfaces?

Let's say I want to compare an algorithm-generated surface to a ground truth surface. So far, I am using overlapping metrics (Dice similarity coefficient), volume and surface distance metrics...

09 December 2013 6,330 15 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to calculate the polysaccharide content of a curcuma species through HPLC method?

what all standards are needed to quantify and analyze the composition with a proper method fie for the mobile phase to run the sample ?

31 January 2021 7,704 4 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to extract polysaccharids from Aloe gel?

I 'm trying to extract polysacharides from Aloe leaf and tried ethanol percipitation, but after getting the pellet. I used the protocol from following reference following the steps, but I don't...

27 January 2021 7,049 5 View

How to measure Tg (Glass Transition) in lyophilised sugar/protein matrices using DSC?

I have been attempting to measure the glass transition temperature of various amorphous powders consisting of sugar/protein mixtures and have had little luck. I typically use a scanning rate of...

13 January 2021 10,022 3 View

Can xylose be formed from fructose over acid catalysts?

I am new in the field of sugar chemistry. I wanted to know if it is possible for fructose to be converted to xylose over acidic conditions. I saw in some schemes a pathway from fructose to xylose...

07 December 2020 5,247 3 View

How to calculate the amount of glucose from the color of Benedict solution?

I'm planning to use Benidict solution to do research on the amount of glucose in candy, but I'm not sure how to calculate the specific amount of sugar from the Benedict solution. Is there a way to...

10 November 2020 1,380 3 View

Can anyone kindly explain me about the chirality of DNA?

As DNA is a macromolecule made up of sugar, phosphates and bases than how can we consider it as a molecule? And secondly which type of experiments we can run to test for the chirality of it? There...

28 October 2020 8,583 3 View

List down the ways one can extract sugar from a fruit / What is the best way to extract sugar from a fruit?

I am conducting research on the Production of Granulated Sugar from Soursop (Annona muricata Linnaeus) Extract. Are there any simple ways I could extract sugar without the use of too many...

27 October 2020 1,349 6 View

Precipitation of sugar contents from Fruit extract?

I have to perform In-vitro and In-vivo anti-diabetic activities on 99%, 70% and 50% ethanolic fruit extracts but I need to precipitate sugar contents first. Can anyone help me with the protocols...

18 October 2020 6,697 5 View