Hi, I wanna know what NaOH type (ready-made solution/ Pellets) y'all use in denaturing libraries when using with MiSeq V2 nano kit and the pH value?

I use a MiSeq machine with MiSeq V2 Nano kit. There isn't any pH value that NaOH should be adjusted to, But I'm wondering if that can cause errors in the sequencing run. Thanks! Eranga

11 June 2018 7,113 2 View

How to do NDVI analysis and data collection?

27 September 2017 1,293 3 View

What kind of metrics can I use to compare two 3D surfaces?

Let's say I want to compare an algorithm-generated surface to a ground truth surface. So far, I am using overlapping metrics (Dice similarity coefficient), volume and surface distance metrics...

09 December 2013 6,330 15 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

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Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Possible reasons for high bioavailability after I.M and S.C. routes...?

What should be the possible reasons if we obtained more than 100% bioavailability of antibiotics (marbofloxacin) after I.M. and S.C. routes of administration in cattle species.

16 February 2021 971 4 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View