I want to visualize the interaction between a receptor protein and its fluorescently labeled partner but receptor-mediated endocytosis or other uptake mechanisms are so intense I cannot observe any membrane structures. How could I decrease endocytosis? Should I lower the temperature I use for the labelling? I do not want inhibit it just decelerate it. What do you think?
Hi Zsóka Csorba ,
You need to use different blockers to inhibit the endocytosis pathways, then you can clearly see the changes in membrane structure. if cells are using so many pathways at the same time, so it is difficult to observe the structures. Yes, temperature affects the overall endocytosis, but you can't see which pathways they are following (clathrin, phagocytosis, micropinocytosis, caveolae, or any other.